Ty1-copia group retrotransposons as ubiquitous components of plant genomes.
نویسندگان
چکیده
منابع مشابه
Sequence evolution and copy number of Ty1-copia retrotransposons in diverse plant genomes.
Sequence evolution of the reverse transcriptase (RT) gene in retrotransposons belonging to the Ty1-copia class was studied in 11 plant species. Phylogenetic reconstruction of the evolutionary history of RT sequences indicated a strong pattern of purifying selection, manifested as high ratios of third to first plus second codon position substitutions, and low ratios of nonsynonymous substitution...
متن کاملTy1-copia group retrotransposons are ubiquitous and heterogeneous in higher plants.
We have used the polymerase chain reaction to isolate fragments of Ty1-copia group retrotransposons from a wide variety of members of the higher plant kingdom. 56 out of 57 species tested generate an amplified fragment of the size expected for reverse transcriptase fragments of Ty1-copia group retrotransposons. Sequence analysis of subclones shows that the PCR fragments display varying degrees ...
متن کاملActivation of Ty1-copia Group Retrotransposons of Dendrobium officinale under Abiotic Stress Conditions
By using universal primer Ty1-copia retrotransposon reverse transcriptase (RT), the conserved reverse transcriptase domain of about 260 bp, which was induced by cold stress and osmotic stress, was amplified by RTPCR from the Dendrobium officinale in this study, indicating that the retrotransposon could be activated by stress conditions. The amplicons were recovered and cloned for sequencing and...
متن کاملHeterogeneity in Ty1-copia group of retroelements in chickpea (
Retrotransposons constitute a major fraction of plant genomes and these elements may have played a significant role in evolution and sequence organization of genomes. In order to access the diversity of Ty1-copia group of retroelements, reverse transcriptase (RT) sequences were amplified from chickpea genome, using the primers derived from two conserved domains of RT region. Thirty-six RT regio...
متن کاملRapid isolation of plant Ty1-copia group retrotransposon LTR sequences for molecular marker studies.
The terminal sequences of long-terminal repeat (LTR) retrotransposons are a source of powerful molecular markers for linkage mapping and biodiversity studies. The major factor limiting the widespread application of LTR retrotransposon-based molecular markers is the availability of new retrotransposon terminal sequences. We describe a PCR-based method for the rapid isolation of LTR sequences of ...
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ژورنال
عنوان ژورنال: The Japanese Journal of Genetics
سال: 1993
ISSN: 0021-504X,1880-5787
DOI: 10.1266/jjg.68.35